Tightly packed monolayers would require some trituration to produce one-cell suspensions. Avoid excessive trituration or shear force by resting the pipette suggestion against the plate edge to take care of viability. If cells do not dissociate perfectly, incubation time with dissociation reagent may possibly must be optimized.
Once the run is finish, retrieve the Shipping and delivery Cartridge from your instrument. The cell sample should be at the bottom or side of the gathering tube. Notice: It is recommended to spin down the Supply Cartridge in the mini-centrifuge for the several seconds for whole volume recovery.
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Cross-part histology accompanied by hematoxylin and eosin (H&E) staining to evaluate the thickness of the compact or large airway epithelium
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Differentiation into adipocytes, osteoblasts, or chondrocytes with appropriate MesenCult™ differentiation systems
TEER measurements is usually carried out repeatedly, devoid of producing damage to the cell lifestyle. It is possible to conduct a weekly TEER time course to explain the barrier perform throughout the entire process of ALI culture differentiation.
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When including GCDR on to the cultures, pipette up and down many situations to mechanically break up the dome totally to ensure GCDR can act on cells for dissociation.
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This is often an stop-place protocol made to dissociate PSC-derived kidney organoids into an individual-cell suspension and fixation for downstream stream cytometry Examination.
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